Effect of cold shock on enzyme release in buffalo ( Bubalus bubalis ) spermatozoa
نویسندگان
چکیده
We have studied the effect of cold shock on the percentage of enzyme release in buffalo spermatozoa, i.e. alcohol, malic, glucose-6-phosphate, isocitric, lactate, sorbital dehydrogenases, glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), hyaluronidase, arylsulphatase A and arylsulphatase B. Significant amounts of alcohol, malic and sorbital dehydrogenases, GOT, hyaluronidase, arylsulphatase A and arylsulphatase B were liberated ; glucose-6-phosphate, isocitric, lactate dehydrogenase and GPT were not affected by cold shock.
منابع مشابه
Reproductive characteristics and thyroidal function in relation with season in Khuzestan buffalo (Bubalus bubalis) bulls
High ambient temperature is the major constraint on Buffalo productivity. The aim of this study was to evaluate the reproductive performance and thyroid gland function in winter and summer seasons in Khuzestan buffalo bulls. Six male indigenous buffaloes of Khuzestan with nearly the same age (2-3 years old) and weight were used. Semen and blood samples through jugular vein were collected, every...
متن کاملOn the leakage of acrosomal hyaluronidase from the spermatozoa of the buffalo (Bubalus bubalis).
The sonication method produced a quantitatively higher release of buffalo sperm hyaluronidase than the freeze-thaw technique. The released enzyme constituted 10 p. 100 of the total enzymatic activity of the fresh semen. Seminal plasma hyaluronidase activity was not correlated with the motility score of the semen sample. Unlike cattle semen, the seminal plasma enzyme level in buffalo semen store...
متن کاملFactors affecting the quality of cryopreserved buffalo (Bubalus bubalis) bull spermatozoa.
Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. Fertility rate in buffalo following artificial insemination with frozen-thawed semen is reviewed. To better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. Moreover, the maj...
متن کاملEffect of Leptin on In Vitro Nuclear Maturation and Apoptosis of Buffalo (Bubalus bubalis) Oocyte
متن کامل
Effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (Bubalus bubalis)
The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 μg mL-1 sodium selenite and the sperm motility and...
متن کامل